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sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page analysis sodium dodecyl sulfate polyacrylamide gel electrophoresis  (Bio-Rad)


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    Structured Review

    Bio-Rad sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page analysis sodium dodecyl sulfate polyacrylamide gel electrophoresis
    Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis Sds Page Analysis Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 2918 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sds+polyacrylamide+gel/Mini-PROTEAN+Tetra+Cell/10__1007_slash_s10068___026___02308___4-83-0-20
    Average 97 stars, based on 2918 article reviews
    sodium dodecyl sulfate polyacrylamide gel electrophoresis sds page analysis sodium dodecyl sulfate polyacrylamide gel electrophoresis - by Bioz Stars, 2026-10
    97/100 stars

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    Expressing:

    Article Title: A nucleotide code governs Lis1's ability to relieve dynein autoinhibition.
    Article Snippet: Separated protein was transferred to PVDF (in 25 mM Tris, 192 mM glycine supplemented with 0.05% SDS and 10% methanol), which was blocked (TBS supplemented with 5% milk) then probed with a monoclonal anti-GFP antibody (1:200; Abm) followed by a horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (at 1:10,000; Jackson ImmunoResearch Laboratories). .. Nature Chemical Biology Article https://doi.org/10.1038/s41589-025-02096-8 Expression levels of WT and mutant Pac1 (both tagged with 13Myc) were determined by loading an equal amount of clarified cell extracts (prepared as described above for Dyn1) directly onto a 4–20% SDS polyacrylamide gel (Bio-Rad). .. After transferring to PVDF, membranes were blocked (as above) and then probed with a rabbit anti-c-Myc antibody (1:500; GenScript) followed by an HRP-conjugated goat anti-rabbit antibody (at 1:10,000; Jackson ImmunoResearch Laboratories).

    Mutagenesis:

    Article Title: A nucleotide code governs Lis1's ability to relieve dynein autoinhibition.
    Article Snippet: Separated protein was transferred to PVDF (in 25 mM Tris, 192 mM glycine supplemented with 0.05% SDS and 10% methanol), which was blocked (TBS supplemented with 5% milk) then probed with a monoclonal anti-GFP antibody (1:200; Abm) followed by a horseradish peroxidase (HRP)-conjugated goat anti-mouse antibody (at 1:10,000; Jackson ImmunoResearch Laboratories). .. Nature Chemical Biology Article https://doi.org/10.1038/s41589-025-02096-8 Expression levels of WT and mutant Pac1 (both tagged with 13Myc) were determined by loading an equal amount of clarified cell extracts (prepared as described above for Dyn1) directly onto a 4–20% SDS polyacrylamide gel (Bio-Rad). .. After transferring to PVDF, membranes were blocked (as above) and then probed with a rabbit anti-c-Myc antibody (1:500; GenScript) followed by an HRP-conjugated goat anti-rabbit antibody (at 1:10,000; Jackson ImmunoResearch Laboratories).

    Membrane:

    Article Title: Overexpression of the erythropoietin receptor is associated with upregulation of tubulin beta 6 and paclitaxel resistance in ovarian adenocarcinoma cells.
    Article Snippet: Protein concentration was determined using a detergent-compatible protein assay (Bio-Rad Laboratories, Hercules, CA, USA). .. Equal amounts of protein (30 μg), supplemented with 0.01% bromophenol blue, 1% 2-mercaptoethanol, 0.4% SDS, and 5% glycerol, were separated on a 10% SDS-polyacrylamide gel (acrylamide/bis solution, 37.5:1) and transferred onto a nitrocellulose (NC) membrane (Bio-Rad Laboratories) via dry transfer for 10 minutes using the iBlot 2 Gel Transfer Device (ThermoFisher, USA). ..

    Article Title: Effect of Denervation on Skeletal Muscle Mitochondria in Heterozygous mtDNA Mutator Mice
    Article Snippet: Before SDS‐PAGE, an aliquot of the extracted protein solution was mixed with an equal volume of sample loading buffer (Bio‐Rad Laboratories, Hercules, CA, USA) containing 1% (vol/vol) 2‐mercaptoethanol. .. The mixture was heated at 37°C for 30 min. Then, 10 μg of protein was separated on an SDS‐polyacrylamide gel and transferred to an ImmunoBlot PVDF membrane (Bio‐Rad Laboratories, Hercules, CA, USA). .. The blot was blocked with Blocking One (Nakalai Tesque Inc., Kyoto, Japan) for 1 h at room temperature, followed by overnight incubation with primary antibodies at 4°C in TBS with 0.1% Tween‐20.

    Article Title: Recombinant AAV vectors for treating neurodegenerative disorders
    Article Snippet: 72 hours after transfection, the supernatant was centrifuged at 2,000 rpm for 10 minutes to remove debris in the medium, 40 μL of supernatant and 10 μL 5× loading buffer (Beyotime, P0015L) were then transferred into a new 1.5 mL tube and incubated for 5 min at 95° C. Cells were washed once with 1×PBS and harvested in the RIPA buffer (Thermo, 89901). .. To conduct WB analysis, the samples were diluted and boiled in 5× loading buffer (Beyotime, P0015L), separated on SDS polyacrylamide gel (BIO-RAD, 1703932) and transferred onto a 0.22 μm PVDF membrane (BIO-RAD, 1620177). .. The membrane was incubated with antibodies for CDNF protein (Sigma, HPA044587), AADC protein (Merck Millipore, AB1569), and β-Tubulin protein (Tubulin, Proteintech, 66240-1-Ig) overnight at 4° C., followed by 2 hours incubation with HRP-linked secondary antibody at room temperature the next day.

    Isolation:

    Article Title: Structural basis for the mechanism and stability of the EEPD1 5′ endonuclease
    Article Snippet: .. The isolated Flag-EEPD1 full length WT sample was loaded onto an SDS polyacrylamide gel (12% Mini-PROTEAN TGX, Bio-Rad Laboratories Inc) and electrophoreses to a distance of 1 cm and stained with Coomassie Brilliant Blue R-250. .. The entire lane was excised, cut into 1 mm pieces and digested overnight with proteomics-grade trypsin (Pierce Trypsin Protease, MS-Grade, ThermoFisher Scientific) following the manufacturer’s recommended protocol.

    Staining:

    Article Title: Structural basis for the mechanism and stability of the EEPD1 5′ endonuclease
    Article Snippet: .. The isolated Flag-EEPD1 full length WT sample was loaded onto an SDS polyacrylamide gel (12% Mini-PROTEAN TGX, Bio-Rad Laboratories Inc) and electrophoreses to a distance of 1 cm and stained with Coomassie Brilliant Blue R-250. .. The entire lane was excised, cut into 1 mm pieces and digested overnight with proteomics-grade trypsin (Pierce Trypsin Protease, MS-Grade, ThermoFisher Scientific) following the manufacturer’s recommended protocol.

    Article Title: Pregnancy negates thyroid hormone-induced pyrexia.
    Article Snippet: .. Proteins were separated on a 10% or 12% SDS-polyacrylamide gel (Bio-Rad TGX Stain-FreeTM 152 FastCastTM, #1610183/5, Germany) and transferred onto polyvinylidene fluoride (PVDF) membranes 153 (Merck Millipore, #05317, Germany). .. Membranes were incubated with the following primary antibodies: 154 rabbit anti-AKT (#9272, RRID:AB_329827), rabbit anti-phospho-AKT (#4060, RRID:AB_2315049), rabbit 155 anti-FoxO1 (#2880, RRID:AB_2106495), rabbit anti-HSL (#4107, RRID:AB_2296900), rabbit anti-phospho-156 HSL (#4126, RRID:AB_490997), rabbit anti-SERCA2 (#4388, RRID:AB_2227684), all from Cell Signalling 157 Technologies in 1:1000 dilution, mouse anti-OxPhos antibody cocktail (1:2,000, Thermo Fisher Scientific 158 #45-8099, RRID:AB_2533835), rabbit anti-UCP1 polyclonal antibody (1:10,000, Merck Millipore, 159 #AB3036, RRID:AB_91297).

    Western Blot:

    Article Title: Effect of Denervation on Skeletal Muscle Mitochondria in Heterozygous mtDNA Mutator Mice
    Article Snippet: Before SDS‐PAGE, an aliquot of the extracted protein solution was mixed with an equal volume of sample loading buffer (Bio‐Rad Laboratories, Hercules, CA, USA) containing 1% (vol/vol) 2‐mercaptoethanol. .. The mixture was heated at 37°C for 30 min. Then, 10 μg of protein was separated on an SDS‐polyacrylamide gel and transferred to an ImmunoBlot PVDF membrane (Bio‐Rad Laboratories, Hercules, CA, USA). .. The blot was blocked with Blocking One (Nakalai Tesque Inc., Kyoto, Japan) for 1 h at room temperature, followed by overnight incubation with primary antibodies at 4°C in TBS with 0.1% Tween‐20.

    Article Title: Recombinant AAV vectors for treating neurodegenerative disorders
    Article Snippet: 72 hours after transfection, the supernatant was centrifuged at 2,000 rpm for 10 minutes to remove debris in the medium, 40 μL of supernatant and 10 μL 5× loading buffer (Beyotime, P0015L) were then transferred into a new 1.5 mL tube and incubated for 5 min at 95° C. Cells were washed once with 1×PBS and harvested in the RIPA buffer (Thermo, 89901). .. To conduct WB analysis, the samples were diluted and boiled in 5× loading buffer (Beyotime, P0015L), separated on SDS polyacrylamide gel (BIO-RAD, 1703932) and transferred onto a 0.22 μm PVDF membrane (BIO-RAD, 1620177). .. The membrane was incubated with antibodies for CDNF protein (Sigma, HPA044587), AADC protein (Merck Millipore, AB1569), and β-Tubulin protein (Tubulin, Proteintech, 66240-1-Ig) overnight at 4° C., followed by 2 hours incubation with HRP-linked secondary antibody at room temperature the next day.



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    Purification and biophysical characterization of EEPD1 . A , domain architecture of human EEPD1. B , Coomassie-stained SDS-PAGE gels showing the purified full-length EEPD1 and its nuclease domain, EEPD1 N uc (aa 261–569). C , chromatograms of the final Size-exclusion chromatography (SEC) chromatograms of full-length EEPD1 and EEPD1 N uc . A total of 3 mg of each protein was loaded onto the columns. D , SEC-MALS traces of EEPD1 and EEPD1nuc domain confirming that both proteins form a stable dimers in solution. E , SEC-MALS analysis demonstrating that EEPD1 dimerization is maintained in a reducing environment (2.0 mM DTT).

    Journal: The Journal of Biological Chemistry

    Article Title: Structural basis for the mechanism and stability of the EEPD1 5′ endonuclease

    doi: 10.1016/j.jbc.2026.111432

    Figure Lengend Snippet: Purification and biophysical characterization of EEPD1 . A , domain architecture of human EEPD1. B , Coomassie-stained SDS-PAGE gels showing the purified full-length EEPD1 and its nuclease domain, EEPD1 N uc (aa 261–569). C , chromatograms of the final Size-exclusion chromatography (SEC) chromatograms of full-length EEPD1 and EEPD1 N uc . A total of 3 mg of each protein was loaded onto the columns. D , SEC-MALS traces of EEPD1 and EEPD1nuc domain confirming that both proteins form a stable dimers in solution. E , SEC-MALS analysis demonstrating that EEPD1 dimerization is maintained in a reducing environment (2.0 mM DTT).

    Article Snippet: The isolated Flag-EEPD1 full length WT sample was loaded onto an SDS polyacrylamide gel (12% Mini-PROTEAN TGX, Bio-Rad Laboratories Inc) and electrophoreses to a distance of 1 cm and stained with Coomassie Brilliant Blue R-250.

    Techniques: Purification, Staining, SDS Page, Size-exclusion Chromatography